New fluorescent cytidine 5'-phosphate derivatives
نویسندگان
چکیده
منابع مشابه
Phosphatidylcholine biosynthesis in castor bean endosperm : purification and properties of cytidine 5'-triphosphate:choline-phosphate cytidylyltransferase.
Cytidine 5'-triphosphate:choline-phosphate cytidylyltransferase (EC 2.7.7.15) has been purified to near homogeneity (3350-fold) from castor bean (Ricinus communis L. var Hale) endosperm. The steps of purification included a differential solubilization of this enzyme with n-octyl beta-d-glucopyranoside (OGP) and column chromatography on sequential DEAE-sepharose, sepharose-6B, and second DEAE-se...
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Calf liver CTP synthetase was purified 2000-fold from cytosol. The formation of 1 mol of CTP from UTP was accompanied by the cleavage of 1 mol of ATP to ADP and the formation of 1 mol of L-glutamate from L-glutamine. The stoichiometry of the liver enzyme reaction was identical with that found for the Escherichia coli B enzyme (Levitzki, A., and Koshland, D. E., Jr. (1971) Biochemistry 10, 3365-...
متن کاملEfficient Deamination of 5-Methylcytidine and 5-Substituted Cytidine Residues in DNA by Human APOBEC3A Cytidine Deaminase
Deamination of 5-methylcytidine (5MeC) in DNA results in a G:T mismatch unlike cytidine (C) deamination which gives rise to a G:U pair. Deamination of C was generally considered to arise spontaneously. It is now clear that human APOBEC3A (A3A), a polynucleotide cytidine deaminase (PCD) with specificity for single stranded DNA, can extensively deaminate human nuclear DNA. It is shown here that A...
متن کاملStructure of Staphylococcus aureus cytidine monophosphate kinase in complex with cytidine 5′-monophosphate
The crystal structure of Staphylococcus aureus cytidine monophosphate kinase (CMK) in complex with cytidine 5'-monophosphate (CMP) has been determined at 2.3 angstroms resolution. The active site reveals novel features when compared with two orthologues of known structure. Compared with the Streptococcus pneumoniae CMK solution structure of the enzyme alone, S. aureus CMK adopts a more closed c...
متن کاملA crystalline fragment of the double helix: the structure of the dinucleoside phosphate guanylyl-3',5'-cytidine.
The sodium salt of guanylyl-3',5'-cytidine crystallizes in a monoclinic unit cell with one molecule in the asymmetric unit. Each molecule is related to another molecule by a 2-fold rotation axis which results in the formation of an antiparallel, right-handed double helix with complementary hydrogen bonding between the guanine and cytosine residues. The crystal is heavily hydrated with 36 water ...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1976
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/3.5.1341